FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (1 ): 27-30.doi: 10.7506/spkx1002-6630-201101007

• Basic Research • Previous Articles     Next Articles

in vitro Antioxidant Activity Evaluation of Elution Fractions of Ethanol Extract from Eucommia ulmoides Leaves on XDA-8 Type Macroporous Resin

DONG Wen-bin1,2，LIU Di2,*，YANG Jin1，XU Xian-meng1   

  1. 1. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China；
    2. College of Chemistry and Chemical Industry, Shaanxi University of Science and Technology, Xi’an 710021, China
  • Received:2010-04-02 Online:2011-01-15 Published:2010-12-28
  • Contact: LIU Di E-mail:didi198012102163.com

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Abstract:

The crude ethanol extract from the leaves of Eucommia ulmoides was applied to XDA-8 type macroporous resin, followed by gradient elution with different concentrations (20%, 40%, 60% and 80%) of ethanol, and four fractions were obtained. The abilities of the four fractions to scavenge hydroxyl, superoxide anion and DPPH free radicals were measured. Meanwhile, the contents of polyphenols and total flavonoids in them were determined. It was found that all the four fractions had excellent scavenging effect on hydroxyl, superoxide anion and DPPH free radicals in a dose-dependent way. This biological activity might be related to total flavonoids and polyphenols, and polyphenols play a leading role. After eluting XDA-8 type macroporous resin with different concentrations of ethanol, eluates were obtained as antioxidants with different chemical composition.

Key words: Eucommia ulmoides leaves, resin, separation and purification, antioxidant activity, free radical

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